铁死亡激活被认为是治疗癌症的有力武器，增强肿瘤细胞对铁死亡的敏感性越来越受到重视。然而，某些铁死亡抵抗机制长期以来受到忽视，特别是肿瘤细胞的异常代谢。
 

图1
 

2023年6月5日，山东大学药学院翟光喜教授、杨小叶副研究员在国际期刊ACS Nano（Q1区，IF=17.1）杂志上发表了题为“A Vanadium-Based Nanoplatform Synergizing Ferroptotic-like Therapy with Glucose Metabolism Intervention for Enhanced Cancer Cell Death and Antitumor Immunity”的研究性论文。

作者提出了一种通过调节肿瘤细胞糖代谢来增强铁死亡激活的模式，并构建了一种名为PMVL的纳米平台。
 

图2 本研究的示意图
 

该平台由负载氯尼达明(LND)的单宁酸与钒氧化物配位而成，并用PD-L1抑制肽修饰的肿瘤细胞膜包裹。

本研究表明，PMVL中钒具有混合价态(V^IV和V^V)，由于V的自循环价态改变而引发铁死亡，这一过程产生•OH用于脂质过氧化积累(V^IV→V^V)，并消耗谷胱甘肽(GSH)用于谷胱甘肽过氧化物酶(GPX4)失活(V^V→V^IV)。值得注意的是，LND通过抑制戊糖磷酸途径(减少NADPH产生)来增强铁死亡，抑制GSH合成。

此外，糖酵解抑制减少了胞内乳酸的产生，减轻了肿瘤微环境的酸性，抑制了免疫抑制性M2巨噬细胞极化。体外和体内结果表明糖代谢干预增强了肿瘤的铁死亡和免疫激活，显著提高了肿瘤的协同治疗效果。

 

联科生物产品助力科研

图3 TNF-α (A), IFN-γ (B), IL-6 (C) 和 IL-2 (D) 的浓度

这篇论文所使用的联科生物产品——Mouse TNF-α ELISA Kit、Mouse IFN-γ ELISA Kit、Mouse IL-6 ELISA Kit和Mouse IL-2 ELISA Kit，广泛应用于科研研究，基础医学，临床研究等领域，如细胞药物研究、免疫治疗领域、干细胞应用领域、肿瘤治疗及靶点药物研究、炎症或病毒感染治疗及预后研究。
 

货号	产品名称	部分引用文献
EK282	Mouse TNF-α ELISA Kit	Multifunctional biomimetic nanoplatform based on photodynamic therapy and DNA repair intervention for the synergistic treatment of breast cancer.
		ASPS Exhibits Anti-Rheumatic Effects by Reprogramming Gut Microbiota and Increasing Serum γ-Glutamylcysteine Level.
		Tumor-Selective Activation of Toll-Like Receptor 7/8 Agonist Nano-Immunomodulator Generates Safe Anti-Tumor Immune Responses upon Systemic Administration.
EK280	Mouse IFN-γ ELISA Kit	Multifunctional biomimetic nanoplatform based on photodynamic therapy and DNA repair intervention for the synergistic treatment of breast cancer.
		Tumor-Selective Activation of Toll-Like Receptor 7/8 Agonist Nano-Immunomodulator Generates Safe Anti-Tumor Immune Responses upon Systemic Administration.
		A therapeutic DC vaccine with maintained immunological activity exhibits robust anti-tumor efficacy.
EK206	Mouse IL-6 ELISA Kit	An injectable liposome-anchored teriparatide incorporated gallic acid-grafted gelatin hydrogel for osteoarthritis treatment.
		Multifunctional biomimetic nanoplatform based on photodynamic therapy and DNA repair intervention for the synergistic treatment of breast cancer.
		Multifunctional Nanostructure Rap-Rl Rescues Alzheimer's Cognitive Deficits through Remodeling the Neurovascular Unit.
EK202	Mouse IL-2 ELISA Kit	An Injectable Hydrogel Reshaping Adenosinergic Axis for Cancer Therapy.
		Artificial Mini Dendritic Cells Boost T Cell-Based Immunotherapy for Ovarian Cancer.
		Separable Microneedle Patch to Protect and Deliver DNA Nanovaccines against Covid-19.

 

参考文献：

Yu Zhang, Xiyou Du, Zhijing He, Shan Gao, Lei Ye, Jianbo Ji, Xiaoye Yang, and Guangxi Zhai. ACS Nano 2023 17 (12), 11537-11556. DOI: 10.1021/acsnano.3c01527.

原文链接：

http://pubs.acs.org/doi/10.1021/acsnano.3c01527